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研究生: 黃滿貞
Man-Chen Huang
論文名稱: 臺灣產芽枝黴屬分離、培養與觀察之研究
Isolation,cultivation and observation of the genus Blastocladia in Taiwan
指導教授: 簡秋源
Chien, Chiu-Yuan
學位類別: 碩士
Master
系所名稱: 生命科學系
Department of Life Science
論文出版年: 2000
畢業學年度: 88
語文別: 中文
論文頁數: 84
中文關鍵詞: 芽枝黴布寧芽枝黴多枝芽枝黴球狀芽枝黴斯帕羅芽枝黴厚芽枝黴
英文關鍵詞: Blastocladia, Blastocladia pringsheimii Reinsch, Blastocladia ramosa Thaxter, Blastocladia globosa Kanouse, Blastocladia sparrowii Indoh, Blastocladia incrassata Indoh
論文種類: 學術論文
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  • 芽枝黴屬(Blastocladia)為壺菌群(Chytridiomycota)之一成員。一般在半停滯或緩流水域中的小樹枝與果實上營腐生,於皮孔上形成半球狀的波狀菌突(crisp hemispherical pustule)。菌體無隔板及假隔板,以假根附著在基質上,細胞壁成分為聚糖-幾丁質。游孢子囊具有單一釋放乳突,游孢子具後生單一鞭毛,並有核冠。本實驗選取蘋果、李子及蕃茄等餌物放置於臺北市、台南市、高雄縣及高雄市共八處水池,經過5至14天後取回實驗室,以1/2GY5培養基做分離、純培養,並以GY5培養基之菌體形態為鑑定依據。實驗結果共獲五菌種如下:
    布寧芽枝黴 (Blastocladia pringsheimii Reinsch)
    多枝芽枝黴 (Blastocladia ramosa Thaxter)
    球狀芽枝黴 (Blastocladia globosa Kanouse)
    斯帕羅芽枝黴 (Blastocladia sparrowii Indoh)
    厚芽枝黴 (Blastocladia incrassata Indoh)
    其中球狀芽枝黴(Blastocladia globosa)為世界新純化種,多枝芽枝黴(Blastocladia ramosa)為臺灣新記錄種及新純化種。

    The genus Blastocladia is a member of the Chytridiomycota. These organisms are predomintly saprophytes on decaying twigs and fruits in semi-stagnant or slowly flowing fresh water on which they form crisp hemispherical pustules. Thallus is tree-like, without true septum, attached by rhizoids to substrata. Cell-wall is non-celluose. No pseudosepta are formed, and sporangia with a exit dehiscence papilla. Zoospores uniciliate posterially, have a nuclear cap. It was successfully cultivated and examined, and identified five species of the genus Blastocladia from Taiwan. They are namely: Bastocladial pringsheimii Reinsch、Bl. ramosa Taxter、Bl. globosa Kanouse、Bl. sparrowii (Sparrow) Indoh and Bl. incrassta Indoh. Bl. globosa is a new axenic culture strains to the taxon (fungal flora). Bl. ramosa is described as a new record and new axenic culture to Taiwain.

    目 錄 目錄…………………………………………………………Ⅰ 圖表目錄……………………………………………………Ⅲ 圖版目錄……………………………………………………Ⅳ 第一章 緒論………………………………………………1 第二章 前人研究…………………………………………5 第三章 材料與方法………………………………………10 3-1 芽枝黴釣集………………………………………10 3-1-1 餌物的預先處理……………………………10 3-1-2 實驗區釣集水域……………………………10 3-1-3 釣集方法……………………………………10 3-2 培養基、酸鹼調節液配製備……………………11 3-2-1 培養基的配製………………………………11 3-2-2 酸鹼調節液的配製…………………………12 3-2-3 漏斗濾紙之製備……………………………12 3-3 芽枝黴分離與純種培養…………………………12 3-4 芽枝黴形態觀察與測量…………………………14 3-4-1 形態學上之觀察……………………………14 3-4-2 形態特徵之測量……………………………15 3-5 芽枝黴菌種之鑑定與保存………………………15 3-5-1 菌種之鑑定…………………………………15 3-5-2 菌種之保存…………………………………16 第四章 結果………………………………………………17 4-1 芽枝黴種的檢索表………………………………17 4-2 芽枝黴種的記述…………………………………19 4-3 芽枝黴形態觀察…………………………………28 4-3-1 布寧芽枝黴…………………………………28 4-3-2 多枝芽枝黴…………………………………30 4-3-3 球狀芽枝黴…………………………………32 4-3-4 斯帕羅芽枝黴………………………………33 4-3-5 厚芽枝黴……………………………………35 第五章 討論………………………………………………38 參考文獻……………………………………………………78 附錄…………………………………………………………83 圖目錄 圖一 鞭毛菌亞群(Mastigomycotina)分類成員簡圖……2 圖二 釣餌放置地點之地理位置分布圖…………………44 表目錄 表一 台灣產芽枝黴五菌株之釣餌放置地點與日期整理表……………………………………………………………45 表二 台灣產五種芽枝黴形態諸特徵……………………46 表三 台灣產五種芽枝黴形態測量總整理表……………47 圖版目錄 圖版Ⅰ 餌物、波狀菌突與多株菌體聚生……………48 圖版Ⅱ 自然基質取下之菌株形態……………………50 圖版Ⅲ 布寧芽枝黴……………………………………52 圖版Ⅳ 布寧芽枝黴……………………………………54 圖版Ⅴ 多枝芽枝黴……………………………………56 圖版Ⅵ 多枝芽枝黴……………………………………58 圖版Ⅶ 多枝芽枝黴……………………………………60 圖版Ⅷ 多枝芽枝黴……………………………………62 圖版Ⅸ 球狀芽枝黴……………………………………64 圖版Ⅹ 球狀芽枝黴……………………………………66 圖版ⅩⅠ 斯帕羅芽枝黴…………………………………68 圖版ⅩⅡ 斯帕羅芽枝黴…………………………………70 圖版XⅢ 厚芽枝黴………………………………………72 圖版ⅩⅣ 厚芽枝黴………………………………………74 圖版ⅩⅤ 厚芽枝黴………………………………………76

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