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研究生: 吳政隆
論文名稱: 利用雙光子顯微術觀察與量化化學增益劑誘使變化在氧化鋅奈米粒子經皮傳輸上
Visualization and Quantification of Chemical Enhancers Induced Changes in Transdermal Zinc Oxide Nanoparticles Transport Using Two-Photon Microscopy
指導教授: 陳家俊
Chen, Chia-Chun
學位類別: 碩士
Master
系所名稱: 化學系
Department of Chemistry
論文出版年: 2008
畢業學年度: 96
語文別: 中文
中文關鍵詞: 氧化鋅奈米粒子雙光子顯微術皮膚滲透
論文種類: 學術論文
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  • 本篇文章目的在於利用雙光子顯微術(TPM)來探討氧化鋅奈米粒子(ZnO NPs)在皮膚上能夠滲透多少以及量化ZnO NPs利用化學增益劑時(如油酸(OA)與乙醇)的滲透增益效應。能夠描述及了解使用了化學增益劑的ZnO NPs在滲透角質層(SC)的經皮傳輸上,能夠用助於設計防曬品的模式。
    TPM為一種非侵入性的方式可用在活體上或活體外人類的皮膚的成像,能直接顯現皮膚結構特徵。有研究指出ZnO具有二階非線性光學係數,觀察到ZnO具有二倍頻訊號(SHG)。利用TPM結合裸鼠皮自發螢光以及ZnO的SHG訊號,能夠用來觀察滲透的路徑與測量ZnO使用與未使用化學性增益劑在SC所滲透的深度。由於化學性增益劑的使用誘使改變ZnO NPs在經皮傳輸的一些性質,包含vehicle to the skin partition coefficient (K), the SHG intensity gradient (dI/dz) and the effective diffusion path length (l),這些將透過三維影像處理來進行理論的分析,評估在裸鼠皮上的效應。
    本實驗中,結果指出(1)ZnO加入與未加入增益劑的滲透深度分別為少於40μm與20μm。(2)ZnO沿角質間隙滲透,最後到達較上層的活化表皮層,而沒有到真皮層。(3)關於傳輸性質的增益值能幫助我們了解增益劑是否有效。

    The aim of this research is using two-photon microscopy (TPM) to visualize how much the skin depth of the zinc oxide nanoparticles (ZnO NPs) penetrating and to quantify the penetration enhancing effect of the ZnO NPs with chemical enhancers (such as Oleic acid - OA and ethanol). A description and understanding of the penetration into the stratum corneum (SC) involved in chemically enhanced transdermal ZnO NPs transport can be used for the design of sunscreen formulations.
    TPM has been used to image in vivo and in vitro human skin as a noninvasive method for directly visualizing the skin structural features. Second-order nonlinear coefficient of ZnO NPs were characterized, and second harmonic generation (SHG) was observed from ZnO NPs. The combination of the autofluorescence of nude mouse skin and the SHG of ZnO NPs has been used to image and measure the penetration pathway and depth of ZnO NPs treated with and without chemical enhancers into the SC. The changes induced by chemical enhancers in the transport properties of the ZnO NPs, including the vehicle to the skin partition coefficient (K), the SHG intensity gradient (dI/dz) and the effective diffusion path length (l), were quantified relative to the control case (without enhancers) utilizing a theoretical analysis of the three-dimensional images for evaluating the effects on nude mouse skin.
    In this work, the results demonstrate that (1) the skin depths of ZnO NPs with and without chemical enhancers penetrating are less than 40μm and 20μm, respectively, (2) ZnO NPs penetrate beyond SC and into the upper viable epidermis, not into the dermis. (3) The enhancements of the transport property are available to understand whether the enhancers induced changes in SC.

    總目錄…………………………………………………………………Ⅰ 中文摘要………………………………………………………………Ⅳ 英文摘要………………………………………………………………Ⅴ 圖目錄…………………………………………………………………Ⅵ 表目錄…………………………………………………………………Ⅷ 第一章 緒論……………………………………………………………1 1-1 引言……………………………………………………………1 1-2 紫外線的傷害…………………………………………………3 1-3 皮膚結構簡介…………………………………………………5 1-3-1 經皮吸收……………………………………………………7 1-3-2 促進劑………………………………………………………7 1-4 防曬品簡介……………………………………………………10 1-5 氧化鋅(ZnO)…………………………………………………12 1-5-1 ZnO發光機制………………………………………………13 1-5-2 ZnO非線性光學性質………………………………………14 1-5-3 奈米ZnO製備方法與機制…………………………………14 1-6 增益劑誘使改變之相關增益係數……………………………18 第二章 實驗……………………………………………………………22 2-1 研究動機與目的………………………………………………22 2-2 奈米ZnO之合成………………………………………………23 2-2-1 合成ZnO nanoparticles與nanorods所需藥品……………23 2-2-2 測量儀器……………………………………………………23 2-2-3 合成ZnO nanoparticles…………………………………… 23 2-2-4 合成ZnO nanorods…………………………………………25 2-2-5 ZnO的octanol-PBS partition coefficient(KO/PBS)測試…… 26 2-2-6 ZnO donor solution配置…………………………………… 27 2-3 皮膚樣品製作…………………………………………………28 2-4 雙光子顯微鏡簡介……………………………………………29 2-5 雙光子顯微鏡儀器與設備……………………………………31 第三章 結果與討論……………………………………………………34 3-1 合成之ZnO材料性質…………………………………………34 3-2 使用雙光子顯微鏡觀察ZnO經皮滲透之影像分析…………38 3-2-1 ZnO的空白測試…………………………………………… 38 3-2-2 ZnO octanol-PBS partition coefficient(KO/PBS)…………… 39 3-2-3 ZnO nanostructures經皮滲透的影像……………………41 3-2-4 ZnO nanoparticles經皮滲透的影像分析…………………43 3-3量化增益劑誘使改變在經皮傳輸ZnO NPs上之增益值………51 第四章 結論與未來展望………………………………………………56 參考文獻………………………………………………………………58

    1. 行政院環境保護署 http://www.epa.gov.tw/

    2. H L Cao, X F Qian, Q Gong, W M Du, X D Ma and Z K Zhu, Nanotechnology, 2006, 17, 3632–3636

    3. Aleksandra B. Djurisˇic’ and Yu Hang Leung, small, 2006, 2, 944–961

    4. Baomei Wen, Yizhong Huang, and John J. Boland, J. Phys. Chem. C, 2008, 112, 106-111.

    5. Peng Jiang, Jian-Jun Zhou, Hai-Feng Fang, Chao-Ying Wang, Zhong Lin Wang, and Si-Shen Xie, Adv. Funct. Mater. 2007, 17, 1303–1310

    6. S.J. Pearton, D.P. Norton, K. Ip, Y.W. Heo, T. Steiner, Progress in Materials Science, 2005, 50, 293–340

    7. 林青郁(2000),人體角質層脂肪含量與藥品滲透性之關係:應用紅外光譜之分析研究,國立成功大學臨床藥學研究所碩士論文

    8 黃美月(1989),皮膚與美容,9~20、45~60、112~120、177~178頁,台北市:聯經

    9. Betty Yu, Chen-Yuan Dong, Peter T. C. So, Daniel Blankschtein, and Robert Langer, THE JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2001, 117, 16-25

    10. JOY HOUK and RICHARD H. GUY, Chemical Reviews., 1988, 88, 457-471

    11. Zhong Lin Wang, Xiang Yang Kong, Yong Ding, Puxian Gao, William L. Hughes, Rusen Yang, Yue Zhang, Adv. Funct. Mater., 2004, 14, 943-956

    12. Changle Wu, Xueliang Qiao, Jianguo Chen, Hongshui Wang, Fatang Tan, Shitao Li, Materials Letters, 2006, 60, 1828–1832

    13. Tai Hwan Ha, Hee-Joon Koo, and Bong Hyun Chung, J. Phys. Chem. C, 2007, 111, 1123-1130.

    14. Ultraviolet Radiation, Environmental Health Criteria 160, World Health Organization Geneva, 1994

    15. Joseph Kushner IV, Daekeun Kim, Reter T.C. So, Daniel Blankschtein and Robert S. Langer, THE JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2007, 127, 2832-2846

    16. Aarti Naik, Louk A,R.M. Pechtold, Russell O. Potts, Richard H. Guy, Journal of Controlled Release, 1995, 37, 299-306

    17. Christelle Ge’ lis, Ste’ phanie Girard, Alain Mavon, Maxence Delverdier, Nicole Paillous1, Patricia Vicendo, Photodermatol Photoimmunol Photomed, 2003, 19, 242–253

    18. A.O. Gamer, E. Leibold, B. van Ravenzwaay, Toxicology in Vitro, 2006, 20, 301–307

    19. De´bora B. Vieira and Ana M. Carmona-Ribeiro, Journal of Antimicrobial Chemotherapy, 2006, 58, 760–767

    20. 劉銘龍 柳中明 黃韋菁(1998),揭開紫外線的奧秘,34-37、頁,台北市:台灣地球日

    21. 邱品齊(2005),保養品真相大公開—美白防曬篇,12~13、84~85、104~105頁,台北市:大境文化
    22. 羅怡情(2005),化妝品成分辭典,157、247頁,台北市:聯經
    23. 駱文(2004),應用雙光子顯微術與化學增益法於觀測量子點滲透皮膚之路徑,國立台灣大學物理學研究所碩士論文。

    24. 孫嬿(2004),多光子偏振及廣義偏振顯微術於皮膚影像上之應用,國立台灣大學物理學研究所碩士論文。

    25. 李今寧(2007),應用雙光子顯微術觀測除毛膏在皮膚角質層之分子穿透增益之影響,國立台灣大學物理學研究所碩士論文。

    26. Buguo Wang, M.J. Callahan, Chunchuan Xu, L.O. Bouthillette, N.C. Giles, D.F. Bliss, Journal of Crystal Growth, 2007, 304, 73–79

    27. D. EzgFr, Ya. I. Alivov, C. Liu, A. Teke, M. A. Reshchikov, S. Dog˘an, V. Avrutin, S.-J. Cho, H. MorkoA, J. Appl. Phys., 2005, 98, 041301.

    28. R. Triboulet, J. Perriere, Prog. Cryst. Growth Charact. Mater.
    , 2003, 47, 65.

    29. A.O. Gamer, E. Leibold, B. van Ravenzwaay, Toxicology in Vitro, 2006, 20, 301-307

    30. J. Schulz, H. Hohemberg, F. Pflucker, E. Gartner, T. Will, S. Pfeiffer, R. Wepf, V. Wendel, H. Gers-Barlag, K.-P. Wittern, Advanced Drug Delivery Reviews 54 Suppl., 2002, 1, S157-S163

    31. C. F. Zhang, Z. W. Dong, G. J. You, R. Y. Zhu, and S. X. Qian, APPLIED PHYSICS LETTERS, 2006, 89, 042117

    32. C. F. Zhang, Z. W. Dong, G. J. You, and S. X. Qian, APPLIED PHYSICS LETTERS, 2005, 87, 051920

    33. Volker Noack and Alexander Eychmuller, Chem. Mater., 2002, 14, 1411-1417

    34. E. A. Meulenkamp, J. Phys. Chem. B, 1998, 102, 5566.

    35. ZHIHONG NIE, DANIELE FAVA, EUGENIA KUMACHEVA, SHAN ZOU, GILBERT C. WALKER AND MICHAEL RUBINSTEIN, nature materials, 2007, 6, 609-614

    36. 陳秀連(2002),以化學法製備均一粒徑氧化鋅粉體與發光特性之研究,國立台灣科技大學/材料科技研究所碩士論文

    37. Elizabeth S. Rowe, Biochemistry, 1983, 22, 3299-3305

    38. Nancy A. Monteiro-Riviere, Alfred O. Inman, Vivien Mak, Philip Wertz, and Jim E. Riviere, Pharmaceutical Research, 2001, 18, 992-998

    39. Leshuai W. Zhang, William W. Yu, Vicki L. Colvin, Nancy A. Monteiro-Riviere, Toxicology and Applied Pharmacology, 2008, 228, 200–211

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